STR/SSR Microsatellite Analysis

︱Product Description    ----------------------------------------------------------------------------------------------------------

Microsatellite Markers (also known as Short Tandem Repeats or Simple Sequence Repeats) are short, repetitive sequences widely distributed throughout eukaryotic genomes. Microsatellite loci are typically analyzed by PCR amplification followed by electrophoretic separation of alleles based on fragment size. The amplified alleles can be detected using ABI genetic analyzers, which utilize fluorescently labeled DNA fragments combined with molecular size standards for precise fragment length calculation. The method supports multiplexed amplification with fluorescent primers, offering high throughput, rapid analysis, high resolution, and suitability for large-scale sample detection.

︱Advantages    ---------------------------------------------------------------------------------------

● Highly Skilled Technical Team

    Supported by a technical team with multiple doctorate-level scientists in molecular biology.

● High-Quality Product Support

    Utilizes premium reagent kits and related products that significantly enhance detection sensitivity while simplifying and accelerating procedures.

● High-Throughput Capability

    Enables multiplexed amplification and detection using primer pairs with different fragment sizes and fluorescent labels, increasing throughput while reducing experimental costs.

● Extensive Detection Experience

    Possesses years of molecular marker detection experience, providing clients with professional technical services.

● Comprehensive Services

    Huaruikang offers preliminary experiments to optimize experimental conditions based on specific client sample characteristics.

︱Services Provided    -------------------------------------------------------------------------------------------------------------

● Microsatellite Experimental Design ：Including amplicon selection and primer design.

● Preliminary Experiments：Optimization and validation of experimental conditions.

● PCR Amplification of genomic DNA.

● Capillary Electrophoresis of PCR amplification products.

● Precise Fragment Sizing and allele calling.

︱Post-Analysis Data Processing    ----------------------------------------------------------------------------------------------

● Genetic Diversity Analysis

    Cluster analysis; diversity analysis; number of polymorphic loci (AP), percentage of polymorphic loci (P), mean number of alleles (Na), effective number of alleles (Ne), Nei's gene diversity index (H), Shannon's information index (I), genetic differentiation coefficient (Gst), etc.

● Genetic Linkage Map Construction

    Utilizes F2, Recombinant Inbred Lines (RIL), CP populations, DH populations, etc., through molecular marker genotyping. Based on genetic recombination test results, the relative positions of markers are calculated to construct chromosome genetic maps.

● Association Analysis

    This involves integrating the genotyping data of detected molecular markers with phenotypic traits to perform population-level statistical analysis. Based on statistical measures or significance p-values, the genetic markers most likely to influence the trait are identified, enabling the discovery of genes associated with trait variation.