Real-Time Fluorescence Quantitative PCR
ProductCategory：Fluorescence Quantification
︱Technical Principle ------------------------------------------------------------------------------------------------------------ Real-Time Fluorescence Quantitative PCR is a molecular technique that utilizes fluorescent reagents within the PCR reaction system to monitor amplification progress in real time. By tracking fluorescence intensity at each cycle, it enables quantitative analysis of the initial template amount through reference genes or standard curve methodology. Real-time PCR employs fluorescent signals to continuously monitor the amplification process during PCR. During the exponential amplification phase, a linear correlation exists between the template's cycle threshold (Ct) value and its initial copy number, forming the foundation for precise quantification. ︱Key Advantages --------------------------------------------------------------------------------------- This technology overcomes the fundamental limitation of traditional PCR by enabling endpoint detection only. Through real-time fluorescence monitoring at every amplification cycle and computerized signal recording, it achieves accurate quantification via Ct value calculation and standard curve analysis. ︱Application Fields ------------------------------------------------------------------------------------------------------------- ● Widely implemented in: ◇ Gene expression studies. ◇ Genetically modified organism research. ◇ Therapeutic efficacy assessment. ◇ Pathogen detection. ◇ Multiple molecular diagnostics domains. |
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