WGBS Comprehensive Accurate Analysis of Genome Methylation: Genome-wide DNA Methylation Sequencing (WGBS) combines bizulfite treatment with Illumina high-throughput sequencing platform to conduct high-precision quasi methylation studies on genome-wide species with reference genomes. WGBS can achieve single base resolution and accurately analyze the methylation status of each cytosine, thus constructing a detailed genome-wide DNA methylation map.
In addition to DNA sequence, the heritable phenotype of an organism is also affected by DNA methylation modification, histone modification, chromatin spatial arrangement and other factors. These patterns of inheritance that do not depend on changing the sequence of DNA to determine biological traits are called epigenetics. Whole genome bisulfite sequencing (WGBS) is a methylation analysis method based on bisulfite. First, the sample DNA was processed by bisulfite to convert the unmethylated C base to the U base, while the methylated C base remained unchanged. Then, combined with high-throughput sequencing technology, a single-base resolution genome-wide DNA methylation map was developed. With the deepening of research, the regulatory mechanism of DNA methylation in biological development and the occurrence of disease has become increasingly clear, and the regulatory network has become increasingly perfect, which also puts forward higher requirements for the study of methylation: comprehensive, single base resolution, combined with multi-omics analysis to improve and refine the regulatory network.
Technical advantages:
1. Accurate results: accurate to a single base, can accurately analyze the methylation state of each C base;
2. Comprehensive detection: complete methylation information can be obtained at the genome-wide level to the maximum extent, and the genome-wide methylation map can be accurately drawn;
3, high applicability: suitable for all species with reference genome, cost-effective.
Research trends and hot spots:
Epigenetic modification changes exist widely in humans and animals and plants. Methylation studies mainly study the influence of epigenetics on phenotypic changes and the mechanism:
1. Study the growth and development of plants and animals: such as different growth stages, different growth periods and different tissues, etc.
2. To study the responses of plants and animals to environmental stress, such as cold resistance, drought resistance, flood resistance, etc.
3. Study the epigenetic mechanism.
4. To study the mechanism of differentiated aging.
5. Study the mechanism of disease development, such as gene methylation caused by cancer.
