QuantStudio 3 and QuantStudio 5Real-Time PCR Systems
|  |  |
| QuantStudio 3 Real-Time PCR System | QuantStudio 5 Real-Time PCR System |
| Sample capacity (wells) | 96 | 96 or 384 |
| Reaction volume | 0.1 mL block: 10–30 μL 0.2 mL block: 10–100 μL | 96-well 0.1 mL block: 10–30 μL 96-well 0.2 mL block: 10–100 μL 384-well block: 5–20 μL |
| Excitation source | Bright white LED |
| Optical detection | 4 coupled fi lters | 96-well: 6 decoupled fi lters 384-well: 5 coupled fi lters |
| Excitation/detection range | 450–600 nm/500–640 nm | 96-well: 450–680 nm/500–730 nm 384-well: 450–650 nm/500–700 nm |
| Multiplexing | Up to 4 targets | 96-well: up to 6 targets 384-well: up to 5 targets |
| Maximum block ramp rate | 0.1 mL block: 9.0°C/sec 0.2 mL block: 6.5°C/sec | 96-well 0.1 mL block: 9.0°C/sec 96-well 0.2 mL block: 6.5°C/sec 384-well block: 6.0°C/sec |
| Average sample ramp rate | 3.66°C/sec |
| Temperature uniformity | 0.4°C |
| Temperature accuracy | 0.25°C |
| Compatible dyes | FAM™/SYBR™ Green, VIC™/JOE™/ HEX™ /TET™, ABY™/NED™/ TAMRA™/Cy®3,JUN™, ROX™/ Texas Red | FAM/SYBR Green, VIC/JOE/HEX/ TET, ABY/NED/TAMRA/Cy3, JUN, ROX/ Texas Red, Mustang Purple™, Cy®5/ LIZ™, Cy®5.5 |
| Security, auditing, and e-signature (SAE) features | No | Yes, with no additional fees |
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