| Instrument | 7500 Fast System | 7500 System |
| Performance |
| Dynamic Range | 9 logs of linear dynamic range |
| Sensitivity | Detection of 1 copy of template in a 20 µL reaction for a single reporter TaqMan ® assay,with 99.7% confidence. | Detection of 1 copy of template in a 50 µL reaction for a single reporter TaqMan ® assay,with 99.7% confidence. |
| Run Time | < 30 minutes | < 2 hours |
| System Specifications |
| Thermal Cycling System | Peltier-based, 96-well block |
| Optical System | CCD camera with halogen lamp excitation;five-excitation and five-emission filters |
| Calibrated Dyes at Installation | FAMTM /SYBR ® Green, VIC ®/JOETM , NEDTM /TAMRATM /Cy3 ®,ROX TM /Texas Red ®, and Cy5 ® |
| Additional Dye Available | Calibration for new dyes within the wavelength rangeis possible by following the custom dye calibrationprocedure in the User’s Manual. Purchase ofadditional filter sets is not necessary. |
| Passive Reference Dyes | ROX dye or any calibrated dye.Use of a passive reference dye is optional. |
| Reaction Volumes | 5–30 µL | 20–100 µL |
| Sample Format | Fast 96-well platesoptimized for 10 µL reactions | Standard 96-well platesand 0.2 mL tubes |
| Peak Block Heating Rate | 5.5°C | 2.5°C |
| Temperature Range | 4–100°C | 4–100°C |
| Temperature Accuracy | +/- 0.25°C of setpoint/display temperature,measured at 3 minutes after clock start |
| Temperature Uniformity | +/- 0.50°C, 30 seconds after clock start |
| Dimensions (w x d x h) | 34 cm (13.99 in) x 45 cm (17.72 in) x 49 cm (19.29 in) |
| Weight | 34 kg (75 lb) |
| Software Specifications |
| Applications | Comparative Ct, Standard Curve, Relative StandardCurve, Allelic Discrimination, Plus/Minus |
| Dye Discrimination | Multicomponenting algorithm |
| Multiplate Data Comparison | Compare an unlimited number plates of geneexpression assays |
| Multiplex Capability | Multiplex up to five targets per well |
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