Instrument Specifications |
Thermal cycling system | Peltier-based, 96-well block |
Sample Ramp Rate | +/-1.1°C/sec |
Peak Block Ramp Rate | 1.6°C/sec |
Temperature Range | 4°C – 100°C |
Temperature Accuracy | ±0.25°C of setpoint/display temperature measuredat 3 minutes after clock start |
Temperature Uniformity | ±0.50°C, 30 seconds after clock start |
Optical system | Single excitation, four emission filters,and CCD camera |
Calibrated Dyes at Installation | SYBR® Green I, FAMTM, VIC®, JOETM, NEDTM,TAMRATM, ROXTM |
Passive Reference Dyes | ROXTM or any calibrated dye. |
Data Collection | Data collected in all 4 filters for all wells regardlessof plate setup. Plate setup may be altered afterrun completes. |
Quantitative PCR run time | < 1 hour 50 minutes |
Supported Volumes | 20 – 100 μL |
Supported Consumables | • Standard optical 96-well plates • 8-strip 0.2mL tubes • 0.2mL tubes • Optical adhesive covers • Optical flat caps |